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AASLD 2015: Sofosbuvir/ Ledipasvir + Vedroprevir Cures 96% of Cirrhotic Genotype 1 Patients


A triple combination of Gilead Sciences' sofosbuvir/ledipasvir (Harvoni) plus the experimental HCV protease inhibitor vedroprevir cured 96% of treatment-experienced, cirrhotic, genotype 1 hepatitis C patients without the need for ribavirin, according to study findings presented at the AASLD Liver Meeting this week in San Francisco.

Interferon-free direct-acting antiviral (DAA) therapy works by targeting different steps of the HCV lifecycle. Combining drugs from different classes produces more potent antiviral activity and reduces the risk of developing drug resistance.

Eric Lawitz from the Texas Liver Institute and colleagues evaluated a regimen consisting of agents from 3 different classes with or without ribavirin. Sofosbuvir is a nucleotide HCV NS5B polymerase inhibitor, ledipasvir is a NS5A inhibitor, and vedroprevir (formerly GS-9451) is an investigational NS3/4A protease inhibitor.

Sofosbuvir/ledipasvir taken alone for 8 weeks is effective for many easier-to-treat people with HCV genotype 1. Some, however, do not achieve sustained response with such a short course of therapy, with prior treatment failure and cirrhosis being among the most important predictors of suboptimal response. This Phase 2 trial assessed whether adding a third DAA could improve response rates for more difficult-to-treat patients and allow them to shorten therapy.

This single-center study included 46 participants with HCV genotype 1, including 70% with harder-to-treat subtype 1a. Two-thirds were men, almost all were white, 87% had unfavorable IL28B non-CC gene variants, and the median age was 57 years. They had compensated cirrhosis without serious functional impairment as reflected in platelet counts and albumin levels. At baseline the mean HCV viral load was about 6 log IU/mL. All had previously been treated with pegylated interferon and ribavirin, but people with prior DAA use were excluded.

Participants in this open-label study were randomly assigned (1:1) to receive sofosbuvir/ledipasvir (400/90 mg coformulation) plus vedroprevir (80 mg) once-daily, either alone or with ribavirin, for 8 weeks. Participants were then followed for 12 more weeks after the end of treatment to determine sustained virological response (SVR12), or continued undetectable HCV RNA.


  • SVR12 rates were 96% in the sofosbuvir/ledipasvir plus vedroprevir arm and 88% in the arm that also added ribavirin.
  • There was 1 relapse in the first arm, and 2 relapses and 1 loss to follow-up in the ribavirin-containing arm.
  • Viral reduction was rapid after starting treatment, with most people having HCV RNA below of the limit of quantification by week 2 -- faster than the decline seen with sofosbuvir/ledipasvir alone.
  • Viral sequencing revealed that 53% of participants had NS3 resistance-associated variants (RAVs) at baseline, while just 7% and 5%, respectively, had NS5A or NS5B RAVs.
  • Presence of NS3 or NS5B RAVs did not have much effect on treatment response, but people with NS5A RAVs were less likely to achieved SVR12 (33% of patients with these RAVs vs 98% of those without).
  • Deep sequencing showed that 2 of the relapsers had the L31M NS5A RAV (associated with reduced susceptibility to ledipasvir) at baseline, and this was enriched post-treatment; 1 of these also had a Q30R variant at baseline but not post-treatment.
  • The third relapser had no RAVs at baseline, but Q30R was detected post-treatment.
  • Treatment with sofosbuvir/ledipasvir plus vedroprevir was generally safe and well-tolerated, with no serious adverse events or discontinuations for this reason.
  • The most common adverse events were headache, rash, sinusitis, cough, and fatigue, all occurring more often in the ribavirin arm.
  • 1 person taking the triple DAA regimen alone and 2 people who added ribavirin developed anemia (hemoglobin <10 g/dL).
  • There were no moderate or worse (> grade 2) ALT or AST liver enzyme elevations.

Ledipasvir/sofosbuvir plus vedroprevir for 8 weeks "achieved high SVR rates in treatment-experienced genotype 1 patients with cirrhosis" and "ribavirin did not enhance SVR rates," the researchers summarized.

Lawitz noted that all 3 relapsers had HCV subtype 1a, but there were no other obvious factors associated with treatment failure. RAVs were the most important predictor, with 2 of the 3 relapsers having dual-class (NS3 and NS5A) RAVs at baseline.

The investigators concluded that "The 3-drug combination of a NS5B nucleotide inhibitor + NS5A inhibitor + NS3 protease inhibitor may allow shortening treatment duration to 8 weeks for patients with more advanced liver disease."



E Lawitz, F Poordad, RH Hyland, et al. High Rates of SVR in Treatment-Experienced Patients with Genotype 1 HCV Infection and Cirrhosis After Treatment with Ledipasvir/Sofosbuvir and Vedroprevir with or Without Ribavirin for 8 weeks. AASLD Liver Meeting 2015. San Francisco, November 13-17, 2015. Abstract 247.