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Vaginal Infections May Help Explain Link Between Hormonal Contraception and Increased HIV Risk


Hormones may alter genital tract immunity in a way that makes women more prone to bacterial and viral infections, while disturbances in natural vaginal microbes may also contribute to the association seen in some studies between use of hormonal contraceptives such as Dep-Provera and susceptibility to HIV infection, according to a laboratory study published in the online journal mBio.

Over the years several studies have found a link between use of hormonal contraception and increased rates of HIV infection, although the association is not consistent and other studies have seen no difference. It is unclear how hormones might increase the risk, though the effect appears to be more pronounced among young women, who have more exposed vulnerable tissue on their cervix than older women.

Raina Fichorova fromHarvard Medical School and colleagues compared immune biomarkers in cervical-vaginal fluid samples from more than 800 reproductive-age Africanwomen using injectable depot medroxyprogesterone acetate (DMPA or Dep-Provera), combined oral contraceptive pills, or no hormonal contraceptives.

They found that women using oral contraceptives or DMPA had differing levels of immune modulators associated with HIV infection, including RANTES and beta-defensin, and that these varied depending on the presence of genital infections including candidiasis, chlamydia, gonorrhea, herpes, and trichomoniasis.

"Thus, the effects of hormonal contraceptives on cervical immunity depend on the genital tract microenvironment, and a weakened mucosal barrier against HIV may be a combined resultant of genital tract infections and HC use," they concluded.

Below is an edited excerpt from a press release issued by the American Society for Microbiology, publisher of mBio, describing the study findings in more detail.

Why Do Certain Hormonal Contraceptives Increase the Risk of HIV?

Washington, DC -- September 1, 2015 -- In recent years, evidence has been building that injectable contraceptive depot medroxyprogesterone acetate (Depo-Provera or DMPA) is associated with an increased risk of HIV infection. Now a study published in the September 1st issue of mBio, an online open-access journal of the American Society for Microbiology, provides a biological explanation for the phenomenon. The findings will help women make more informed choices about birth control.

"Before this study, there were all these controversial reports, some showing that DMPA increases the risk of HIV infection and others showing it doesn't, and there was no biologic explanation for the differences between studies," said lead author Raina Fichorova, PhD, MD, director of the Division of Genital Tract Biology at Brigham and Women's Hospital and associate professor of obstetrics and gynecology and reproductive biology at Harvard Medical School, Boston. "This new study offers an explanation for the inconsistent studies, and it lies in the microbial communities of the reproductive tract."

The researchers analyzed cervical swabs and data from 823 women, between the ages of 18 and 35, who were HIV negative and enrolled in family planning clinics in Uganda and Zimbabwe. Roughly 200 women in this cohort became HIV infected. Women were divided into three groups, those who used DMPA, those who used estrogen-progesterone oral contraceptives, and those who used no hormonal contraceptives. Within each of these groups, the investigators compared results for women with a healthy vaginal environment (dominated by Lactobacillus-morphotypes and free of bacterial vaginosis) to women who had a disturbed vaginal microbioata or an infection from bacteria, fungi or parasites.

The team then looked to see if the women taking oral contraceptives or receiving DMPA were more at risk for immunological changes that can increase a person's vulnerability to HIV infection than women who weren't taking a hormonal contraceptive. They found that DMPA use was associated with an increase in these immunological changes, and that the presence of certain vaginal infections further increased this risk. In addition, women who had certain vaginal infections or disturbed resident microbiota and took oral contraceptives were also at increased risk for this unfavorable, immunological profile.

For example, women who had herpes and took DMPA, as well as women who had herpes or disturbed vaginal microbiota and at the same time took levonorgestrel-containing oral contraceptives, were more likely to have increased levels of proteins that attract HIV host cells. This kind of inflammatory response is implicated in increasing the risk of HIV infection, transmission, or progression.

The research team also found that concurrent infections or disturbed vaginal microbiota may also exacerbate the suppression of the immune system by DMPA, thus adding to a woman's vulnerability to HIV. For example, DMPA appeared to suppress immune responses to Trichomonas vaginalis, a wide-spread parasite that aids HIV infection.

"Women deserve to know more so that they can make informed choices about birth control. Both men and women should be educated about our findings, as both partners are at risk and need to prevent and treat infections," said Dr. Fichorova. "Studies of new contraceptive methods should evaluate how they impact the microbial environment and how they act in concert with preexisting, treatable microbial disturbances, to weaken the mucosal barrier against HIV and other infections. Our hope is to prevent the unwanted side effects of available hormonal contraceptives and improve and save millions of lives by developing new affordable tools and approaches to restore and keep the healthy vaginal microbial environment in women of reproductive age."



RN Fichorova, PL Chen, CS Morrison, C Mauck, et al. The Contribution of Cervicovaginal Infections to the Immunomodulatory Effects of Hormonal Contraception. mBio 6(5):e00221-15. September 1, 2015.

Other Source

American Society for Microbiology. Why Do Certain Hormonal Contraceptives Increase the Risk of HIV? Press release. September 1, 2015.